Molecular cloning of a cDNA encoding the feline CD62L

Yorihiro Nishimura; Masayuki Shimojima; Yukinobu Tohya; Takayuki Miyazawa

doi:10.1292/jvms.69.81

Molecular cloning of a cDNA encoding the feline CD62L

J Vet Med Sci. 2007 Jan;69(1):81-4. doi: 10.1292/jvms.69.81.

Authors

Yorihiro Nishimura¹, Masayuki Shimojima, Yukinobu Tohya, Takayuki Miyazawa

Affiliation

¹ Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

PMID: 17283407
DOI: 10.1292/jvms.69.81

Abstract

We cloned a cDNA fragment encoding a feline homologue of L-selectin (CD62L). The extracellular region of the feline CD62L fragment contained a calcium-dependent (C-type) lectin domain, an epidermal growth factor-like domain, and two Sushi/CCP/SCR domains. The flow cytometric analysis confirmed that the feline CD62L molecule, which was expressed 293T cells, retained an epitope recognized by an anti-human CD62L monoclonal antibody (Leu-8).

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal / metabolism
Base Sequence
Cats / genetics*
Cell Line
Cloning, Molecular
DNA Primers
DNA, Complementary / genetics
Flow Cytometry
Humans
L-Selectin / genetics*
L-Selectin / metabolism*
Molecular Sequence Data
Protein Structure, Tertiary
Sequence Analysis, DNA

Substances

Antibodies, Monoclonal
DNA Primers
DNA, Complementary
L-Selectin