Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

Nicola Heim; Olga Garaschuk; Michael W Friedrich; Marco Mank; Ruxandra I Milos; Yury Kovalchuk; Arthur Konnerth; Oliver Griesbeck

doi:10.1038/nmeth1009

Improved calcium imaging in transgenic mice expressing a troponin C-based biosensor

Nat Methods. 2007 Feb;4(2):127-9. doi: 10.1038/nmeth1009. Epub 2007 Jan 28.

Authors

Nicola Heim¹, Olga Garaschuk, Michael W Friedrich, Marco Mank, Ruxandra I Milos, Yury Kovalchuk, Arthur Konnerth, Oliver Griesbeck

Affiliation

¹ Max Planck Institute of Neurobiology, Am Klopferspitz 18, 82152 Martinsried, Germany.

PMID: 17259991
DOI: 10.1038/nmeth1009

Abstract

Fluorescent Ca(2+) indicator proteins (FCIPs) are attractive tools for studying Ca(2+) dynamics in live cells. Here we describe transgenic mouse lines expressing a troponin C (TnC)-based biosensor. The biosensor is widely expressed in neurons and has improved Ca(2+) sensitivity both in vitro and in vivo. This allows FCIP-based two-photon Ca(2+) imaging of distinct neurons and their dendrites in vivo, and opens a new avenue for structure-function analysis of intact neuronal circuits.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques / methods*
Brain / cytology
Brain / embryology
Brain / metabolism*
Calcium / metabolism*
Calcium Signaling*
Dendrites / metabolism
Female
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Mice, Transgenic
Neurons / metabolism*
Spectrometry, Fluorescence
Troponin C / genetics
Troponin C / metabolism*

Substances

Cyan Fluorescent Protein
Troponin C
Green Fluorescent Proteins
Calcium