Kinin receptors in stimulated and characterized decidua tissue-derived cells

Elisabeth Schremmer-Danninger; Dorit K Nägler; Kerstin Miska; Michael J Flaig; Alexander Faussner; Edwin Fink; Matthias C Raggi; Marianne Jochum; Joachim Rehbock

doi:10.1016/j.intimp.2006.09.002

Kinin receptors in stimulated and characterized decidua tissue-derived cells

Int Immunopharmacol. 2007 Jan;7(1):103-12. doi: 10.1016/j.intimp.2006.09.002. Epub 2006 Oct 3.

Authors

Elisabeth Schremmer-Danninger¹, Dorit K Nägler, Kerstin Miska, Michael J Flaig, Alexander Faussner, Edwin Fink, Matthias C Raggi, Marianne Jochum, Joachim Rehbock

Affiliation

¹ Division of Clinical Chemistry and Clinical Biochemistry in the Department of Surgery, Ludwig-Maximilians-University Munich, Nussbaumstrasse 20, D-80336 Munich, Germany. Schremmer-Danninger@imetum.de

PMID: 17161823
DOI: 10.1016/j.intimp.2006.09.002

Abstract

Bradykinin and its kinin B(2) receptor are autocrine and paracrine mediators in foetal membranes and decidua. As a first step we characterized the intracellular morphology of decidual cells. Cultured decidua tissue-derived cells immunolabel for vimentin fibrils, and are considered to be of mesenchymal origin. They show characteristics of macrophages and can be distinguished from endothelial cells and cells of the trophoblast lineage. These cellular features were determined by means of immunocytochemistry. Furthermore cultured decidua tissue-derived cells express kinin B(2) receptors and in this context we demonstrated its expression at mRNA level by in situ reverse transcriptase polymerase chain reaction. Following stimulation with bacterial lipopolysaccharide, we have observed a marginal upregulation of the expression of kinin B(1) receptors and carboxypeptidase M by quantitative RT-PCR. Equilibrium binding experiments with [(3)H]des-Arg(10)-kallidin, the kinin B(1) receptor agonist, did not result in detectable binding sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / immunology
Antigens, CD / immunology
Antigens, Differentiation, Myelomonocytic / immunology
Decidua / cytology*
Decidua / metabolism*
Female
GPI-Linked Proteins
Humans
Interleukin-1beta / pharmacology
Keratins / immunology
Leukosialin / immunology
Lipopolysaccharides / pharmacology
Metalloendopeptidases / genetics
Metalloendopeptidases / metabolism
Platelet Endothelial Cell Adhesion Molecule-1 / immunology
RNA, Messenger / metabolism
Receptor, Bradykinin B1 / genetics
Receptor, Bradykinin B1 / metabolism
Receptor, Bradykinin B2 / genetics
Receptor, Bradykinin B2 / metabolism*
Vimentin / immunology

Substances

Antibodies, Monoclonal
Antigens, CD
Antigens, Differentiation, Myelomonocytic
CD68 antigen, human
GPI-Linked Proteins
Interleukin-1beta
Leukosialin
Lipopolysaccharides
Platelet Endothelial Cell Adhesion Molecule-1
RNA, Messenger
Receptor, Bradykinin B1
Receptor, Bradykinin B2
Vimentin
Keratins
carboxypeptidase M
Metalloendopeptidases