Abstract
We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine ((CV)U)-containing ODN at the 5'-terminal. This method forms d(T-(CV)U) as a cyclobutane pyrimidine dimer (CPD) analogue between 3'-terminal thymidine and 5'-terminal (CV)U of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-(CV)U). And then, we disclosed that two adenines were incorporated opposite the d(T-(CV)U).
MeSH terms
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Adenine / metabolism
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DNA / biosynthesis*
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DNA / chemistry
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Deoxyuridine / analogs & derivatives*
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Deoxyuridine / chemistry
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Electrophoresis, Microchip
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Oligodeoxyribonucleotides / chemistry
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Oligodeoxyribonucleotides / radiation effects
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Photochemistry
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Polymerase Chain Reaction
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Pyrimidine Dimers / chemistry*
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Taq Polymerase / metabolism*
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Templates, Genetic
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Thymidine / analogs & derivatives*
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Thymidine / chemistry
Substances
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Oligodeoxyribonucleotides
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Pyrimidine Dimers
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DNA
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Taq Polymerase
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Adenine
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Thymidine
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Deoxyuridine