Molecular cloning and characterization of human Aph2 gene, involved in AP-1 regulation by interaction with JAB1

Fengrui Zhang; Yujun Di; Jinjun Li; Yan Shi; Liping Zhang; Chunyan Wang; Xianghuo He; Yongzhong Liu; Dafang Wan; Keke Huo; Jianren Gu

doi:10.1016/j.bbaexp.2006.10.002

Molecular cloning and characterization of human Aph2 gene, involved in AP-1 regulation by interaction with JAB1

Biochim Biophys Acta. 2006 Nov-Dec;1759(11-12):514-25. doi: 10.1016/j.bbaexp.2006.10.002. Epub 2006 Oct 20.

Authors

Fengrui Zhang¹, Yujun Di, Jinjun Li, Yan Shi, Liping Zhang, Chunyan Wang, Xianghuo He, Yongzhong Liu, Dafang Wan, Keke Huo, Jianren Gu

Affiliation

¹ State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute and Cancer Institute of Shanghai Jiaotong University, Shanghai 200032, PR China.

PMID: 17123647
DOI: 10.1016/j.bbaexp.2006.10.002

Abstract

A human Aph2 gene (hAph2) was identified and cloned from a human placenta cDNA library. Bioinformatics analysis revealed hAPH2 protein shares 96% identity with mouse APH2 and contains a zf-DHHC domain (148-210aa), which is always involved in protein-protein or protein-DNA interaction. Differential expression patterns of hAph2 mRNA were observed in normal human tissues. Yeast two-hybrid screening found another hAPH2-interacting protein JAB1. The zf-DHHC domain of hAPH2 and the C-terminal of JAB1 were confirmed to be critical for the interaction. Fused with GFP and expressed in COS-7, NIH/3T3 and SMMC-7721 cell lines, hAPH2 showed predominant distribution in the cytoplasm and co-localized with JAB1 around the nucleus. Furthermore, overexpression of hAPH2 could increase apoptosis of COS-7 cells and negatively regulate JAB1-induced activation of AP-1 in a concentration dependent manner. The expression level of c-jun was also down-regulated by overexpression of hAPH2 in COS-7 cells. These data showed some basic characterization and function of hAph2 (hAPH2), dependent or independent with JAB1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Amyloid Precursor Protein Secretases / genetics*
Amyloid Precursor Protein Secretases / metabolism
Animals
Base Sequence
Binding Sites / genetics
Blotting, Western
COP9 Signalosome Complex
COS Cells
Cell Line, Tumor
Chlorocebus aethiops
Cloning, Molecular
Female
Gene Expression Profiling
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism*
Luciferases / genetics
Luciferases / metabolism
Membrane Glycoproteins / genetics*
Membrane Glycoproteins / metabolism
Mice
Microscopy, Fluorescence
Molecular Sequence Data
NIH 3T3 Cells
Peptide Hydrolases / genetics
Peptide Hydrolases / metabolism*
Protein Binding
Sequence Homology, Amino Acid
Transcription Factor AP-1 / metabolism*
Transfection
Two-Hybrid System Techniques

Substances

Intracellular Signaling Peptides and Proteins
Membrane Glycoproteins
Transcription Factor AP-1
nicastrin protein
Green Fluorescent Proteins
Luciferases
Amyloid Precursor Protein Secretases
Peptide Hydrolases
COPS5 protein, human
COP9 Signalosome Complex

Associated data

GENBANK/AF258563