Mycobacterium monacense sp. nov

Udo Reischl; Holger Melzl; Reiner M Kroppenstedt; Thomas Miethke; Ludmila Naumann; Alessandro Mariottini; Gianna Mazzarelli; Enrico Tortoli

doi:10.1099/ijs.0.64527-0

Mycobacterium monacense sp. nov

Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2575-2578. doi: 10.1099/ijs.0.64527-0.

Authors

Udo Reischl¹, Holger Melzl¹, Reiner M Kroppenstedt², Thomas Miethke³, Ludmila Naumann⁴, Alessandro Mariottini^{5

6}, Gianna Mazzarelli^{7

6}, Enrico Tortoli⁶

Affiliations

¹ Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany.
² DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany.
³ Institute of Medical Microbiology and Hygiene, Technical University of Munich, Munich, Germany.
⁴ Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Oberschleißheim, Germany.
⁵ Cytogenetics and Genetics Unit, Careggi Hospital, Florence, Italy.
⁶ Mycobacteriology Reference Center, Microbiology Laboratory, Careggi Hospital, Florence, Italy.
⁷ Microbiological and Virological Serum-Immunology Laboratory, Careggi Hospital, Florence, Italy.

PMID: 17082393
DOI: 10.1099/ijs.0.64527-0

Abstract

Four bacterial strains were isolated from independent clinical specimens in different countries and their genotypic and phenotypic characters support their classification in a novel species within the genus Mycobacterium. One strain was clearly responsible for a severe, post-traumatic wound infection in a healthy boy. The novel species, for which the name Mycobacterium monacense sp. nov. is proposed, is yellow-pigmented, non-photochromogenic and grows in less than a week on solid medium. Based on phenotypic investigations alone, distinction of these four strains from known scotochromogenic rapidly growing strains is problematic. However, the novel strains differ from any other mycobacterium in each of the molecular species markers investigated: the 16S rRNA gene, the 16S-23S rRNA gene internal transcribed spacer and the hsp65 gene. Of the strains investigated, two different sequevars were detected for the hsp65 region. Phylogenetic analysis revealed that these four strains were most closely related to Mycobacterium doricum. The type strain of Mycobacterium monacense sp. nov. is B9-21-178T (=DSM 44395T=CIP 109237T).

MeSH terms

Adult
Aged, 80 and over
Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
Bronchoalveolar Lavage Fluid / microbiology
Chaperonin 60
Chaperonins / genetics
Child
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
DNA, Ribosomal Spacer / chemistry
DNA, Ribosomal Spacer / genetics
Female
Genes, rRNA
Germany
Humans
Italy
Male
Microbial Sensitivity Tests
Molecular Sequence Data
Mycobacterium Infections, Nontuberculous / microbiology*
Mycolic Acids / analysis*
Nontuberculous Mycobacteria / classification*
Nontuberculous Mycobacteria / isolation & purification*
Nontuberculous Mycobacteria / physiology
Phylogeny
Pigments, Biological
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sputum / microbiology
Wound Infection / microbiology

Substances

Anti-Bacterial Agents
Bacterial Proteins
Chaperonin 60
DNA, Bacterial
DNA, Ribosomal
DNA, Ribosomal Spacer
Mycolic Acids
Pigments, Biological
RNA, Bacterial
RNA, Ribosomal, 16S
heat-shock protein 65, Mycobacterium
Chaperonins

Associated data

GENBANK/AF107039
GENBANK/DQ381726
GENBANK/DQ381730
GENBANK/DQ381731
GENBANK/DQ381732
GENBANK/DQ466891
GENBANK/DQ466892
GENBANK/DQ466893
GENBANK/DQ466894
GENBANK/DQ466895
GENBANK/DQ466896
GENBANK/DQ466897
GENBANK/DQ466898
GENBANK/DQ466899
GENBANK/DQ466900
GENBANK/DQ466901
GENBANK/DQ466902
GENBANK/DQ466903
GENBANK/DQ473393