Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1138-40. doi: 10.1107/S1744309106041959. Epub 2006 Oct 20.

Abstract

A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using polyethylene glycol 8000 as a precipitant and have a thick rod-like morphology. Their crystallographic symmetry is orthorhombic, space group C222(1), with unit-cell parameters a = 57.2, b = 86.2, c = 72.6 A and a diffraction limit of 2.06 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / isolation & purification
  • COP9 Signalosome Complex
  • Crystallography, X-Ray
  • DNA, Complementary
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Multiprotein Complexes / chemistry*
  • Peptide Hydrolases / chemistry*
  • Polymerase Chain Reaction
  • Protein Subunits / chemistry
  • Protein Subunits / isolation & purification*

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • Multiprotein Complexes
  • Protein Subunits
  • Peptide Hydrolases
  • COP9 Signalosome Complex