Characterization and application of aptamers for Taq DNA polymerase selected using an evolution-mimicking algorithm

Biotechnol Lett. 2006 Dec;28(23):1939-44. doi: 10.1007/s10529-006-9178-4. Epub 2006 Sep 19.

Abstract

Using an evolution-mimicking algorithm (EMA), we have recently identified DNA aptamers that inhibit Taq DNA polymerase. In the present study, we have attempted to improve further the inhibitory activities of aptamers, as well as to characterize those aptamers with the most potent inhibitory activities. To characterize the most potent aptamer and demonstrate its applicability, the abilities to inhibit Tth DNA polymerase and to modulate specific amplification in PCR were investigated. This aptamer inhibited both Tth DNA polymerase and Taq DNA polymerase and improved the specificity of detection of a low-copy-number target gene in PCR using these DNA polymerases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Aptamers, Nucleotide*
  • Base Sequence
  • Biotechnology / methods*
  • DNA, Viral / genetics
  • Evolution, Molecular
  • HIV-1 / genetics
  • Ligands
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • Taq Polymerase / chemistry*
  • Taq Polymerase / genetics*

Substances

  • Aptamers, Nucleotide
  • DNA, Viral
  • Ligands
  • Oligonucleotides
  • Taq Polymerase