Abstract
Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Down-regulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Autoantigens / genetics*
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cell Line
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DNA Probes
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Humans
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Leukemia
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Molecular Sequence Data
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Myeloblastin
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Oligodeoxyribonucleotides
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Oligonucleotides, Antisense
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction
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RNA, Messenger / genetics*
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RNA, Messenger / isolation & purification
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Restriction Mapping
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Serine Endopeptidases / genetics*
Substances
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Autoantigens
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DNA Probes
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DNA, Neoplasm
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Oligodeoxyribonucleotides
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Oligonucleotides, Antisense
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RNA, Messenger
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Poly A
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Serine Endopeptidases
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Myeloblastin
Associated data
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GENBANK/M75154
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GENBANK/S60069
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GENBANK/S60071
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GENBANK/S60073
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GENBANK/S60076
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GENBANK/S60079
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GENBANK/S60081
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GENBANK/S60988
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GENBANK/S61137
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GENBANK/S61158