Wegener autoantigen and myeloblastin are encoded by a single mRNA

Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9253-6. doi: 10.1073/pnas.88.20.9253.

Abstract

Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Down-regulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoantigens / genetics*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cell Line
  • DNA Probes
  • DNA, Neoplasm / genetics
  • DNA, Neoplasm / isolation & purification
  • Humans
  • Leukemia
  • Molecular Sequence Data
  • Myeloblastin
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • Poly A / genetics
  • Poly A / isolation & purification
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics*
  • RNA, Messenger / isolation & purification
  • Restriction Mapping
  • Serine Endopeptidases / genetics*

Substances

  • Autoantigens
  • DNA Probes
  • DNA, Neoplasm
  • Oligodeoxyribonucleotides
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Poly A
  • Serine Endopeptidases
  • Myeloblastin

Associated data

  • GENBANK/M75154
  • GENBANK/S60069
  • GENBANK/S60071
  • GENBANK/S60073
  • GENBANK/S60076
  • GENBANK/S60079
  • GENBANK/S60081
  • GENBANK/S60988
  • GENBANK/S61137
  • GENBANK/S61158