Abstract
Aim:
To explore the possible mechanisms of curcumin in rat colitis induced by trinitrobenzene sulfonic (TNBS) acid.
Methods:
Rats with TNBS acid-induced colitis were treated with curcumin (30 mg/kg or 60 mg/kg per day ip). Changes of body weight and histological scores as well as survival rate were evaluated. Leukocyte infiltration was detected by myeloperoxidase (MPO) activity assay. The expression of cyclooxygenase-2 (COX-2) was detected by RT-PCR and Western blot. Inflammation cytokines were determined by RT-PCR. Local concentration of prostaglandin E(2) (PGE(2)) in colon mucosa was determined by ELISA.
Results:
Curcumin improved survival rate and histological image, decreased the macroscopic scores and MPO activity. Also curcumin reduced the expression of COX-2 and inflammation cytokines. In addition, treatment with curcumin increased the PGE(2) level.
Conclusion:
Curcumin has therapeutic effects on TNBS acid-induced colitis, the mechanisms seem to be related to COX-2 inhibition and PGE(2) improvement.
MeSH terms
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Animals
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Body Weight / drug effects
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Body Weight / physiology
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Chronic Disease
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Colitis / chemically induced*
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Colitis / drug therapy*
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Colitis / enzymology
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Colitis / physiopathology
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Colon / chemistry
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Colon / enzymology
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Colon / pathology
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Colon / physiopathology
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Curcumin / pharmacology
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Curcumin / therapeutic use*
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Cyclooxygenase 2 / metabolism*
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Cyclooxygenase 2 Inhibitors / pharmacology
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Cyclooxygenase 2 Inhibitors / therapeutic use*
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Dinoprostone / metabolism
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / physiology
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Interferon-gamma / analysis
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Interferon-gamma / genetics
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Interferon-gamma / physiology
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Intestinal Mucosa / chemistry
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Intestinal Mucosa / enzymology
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Intestinal Mucosa / pathology
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Intestinal Mucosa / physiopathology
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Leukocytes / pathology
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Leukocytes / physiology
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Nitric Oxide Synthase Type II / analysis
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / physiology
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Rats
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Rats, Sprague-Dawley
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Trinitrobenzenesulfonic Acid / adverse effects*
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Tumor Necrosis Factor-alpha / analysis
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / physiology
Substances
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Cyclooxygenase 2 Inhibitors
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Tumor Necrosis Factor-alpha
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Interferon-gamma
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Trinitrobenzenesulfonic Acid
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Nitric Oxide Synthase Type II
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Nos2 protein, rat
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Cyclooxygenase 2
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Curcumin
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Dinoprostone