Curcumin-attenuated trinitrobenzene sulphonic acid induces chronic colitis by inhibiting expression of cyclooxygenase-2

World J Gastroenterol. 2006 Jun 28;12(24):3848-53. doi: 10.3748/wjg.v12.i24.3848.

Abstract

Aim: To explore the possible mechanisms of curcumin in rat colitis induced by trinitrobenzene sulfonic (TNBS) acid.

Methods: Rats with TNBS acid-induced colitis were treated with curcumin (30 mg/kg or 60 mg/kg per day ip). Changes of body weight and histological scores as well as survival rate were evaluated. Leukocyte infiltration was detected by myeloperoxidase (MPO) activity assay. The expression of cyclooxygenase-2 (COX-2) was detected by RT-PCR and Western blot. Inflammation cytokines were determined by RT-PCR. Local concentration of prostaglandin E(2) (PGE(2)) in colon mucosa was determined by ELISA.

Results: Curcumin improved survival rate and histological image, decreased the macroscopic scores and MPO activity. Also curcumin reduced the expression of COX-2 and inflammation cytokines. In addition, treatment with curcumin increased the PGE(2) level.

Conclusion: Curcumin has therapeutic effects on TNBS acid-induced colitis, the mechanisms seem to be related to COX-2 inhibition and PGE(2) improvement.

MeSH terms

  • Animals
  • Body Weight / drug effects
  • Body Weight / physiology
  • Chronic Disease
  • Colitis / chemically induced*
  • Colitis / drug therapy*
  • Colitis / enzymology
  • Colitis / physiopathology
  • Colon / chemistry
  • Colon / enzymology
  • Colon / pathology
  • Colon / physiopathology
  • Curcumin / pharmacology
  • Curcumin / therapeutic use*
  • Cyclooxygenase 2 / metabolism*
  • Cyclooxygenase 2 Inhibitors / pharmacology
  • Cyclooxygenase 2 Inhibitors / therapeutic use*
  • Dinoprostone / metabolism
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Enzymologic / physiology
  • Interferon-gamma / analysis
  • Interferon-gamma / genetics
  • Interferon-gamma / physiology
  • Intestinal Mucosa / chemistry
  • Intestinal Mucosa / enzymology
  • Intestinal Mucosa / pathology
  • Intestinal Mucosa / physiopathology
  • Leukocytes / pathology
  • Leukocytes / physiology
  • Nitric Oxide Synthase Type II / analysis
  • Nitric Oxide Synthase Type II / genetics
  • Nitric Oxide Synthase Type II / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Trinitrobenzenesulfonic Acid / adverse effects*
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / physiology

Substances

  • Cyclooxygenase 2 Inhibitors
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Trinitrobenzenesulfonic Acid
  • Nitric Oxide Synthase Type II
  • Nos2 protein, rat
  • Cyclooxygenase 2
  • Curcumin
  • Dinoprostone