Over-expression and characterization of the recombinant small heat shock protein from Pyrococcus furiosus

Huayou Chen; Zhongmei Chu; Yi Zhang; Shengli Yang

doi:10.1007/s10529-006-9058-y

Over-expression and characterization of the recombinant small heat shock protein from Pyrococcus furiosus

Biotechnol Lett. 2006 Jul;28(14):1089-94. doi: 10.1007/s10529-006-9058-y. Epub 2006 Jun 24.

Authors

Huayou Chen¹, Zhongmei Chu, Yi Zhang, Shengli Yang

Affiliation

¹ Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai, China.

PMID: 16799764
DOI: 10.1007/s10529-006-9058-y

Abstract

The gene encoding a small heat shock protein (sHSP) from Pyrococcus furiosus was redesigned and chemically synthesized by using bacteria-preferred codons. The gene product was over-expressed in Escherichia coli BL21(DE)(3) and purified to homogeneity. In the presence of this protein, the activities of Taq DNA polymerase, DNA restriction endonuclease HindIII and lysozyme were protected at elevated temperature, and also, thermal aggregation of lysozyme was prevented by this purified recombinant sHSP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Activation
Enzyme Stability
Escherichia coli / enzymology*
Escherichia coli / genetics
Heat-Shock Proteins, Small / chemistry*
Heat-Shock Proteins, Small / genetics
Heat-Shock Proteins, Small / metabolism*
Protein Engineering / methods*
Pyrococcus furiosus / enzymology*
Pyrococcus furiosus / genetics
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Up-Regulation / genetics

Substances

Heat-Shock Proteins, Small
Recombinant Proteins