Abstract
To address the function of RNA interference (RNAi) in transcriptional silencing in mammals, we analyzed genomic imprinting in Dicer1-hypomorphic mice, in which Dicer1 expression was significantly reduced. We did not observe any abnormality in the allelic expression of imprinted genes in these mice or their offspring, suggesting that reduced expression of Dicer1 did not significantly affect the maintenance and reprogramming of imprinting.
2006 S. Karger AG, Basel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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DEAD-box RNA Helicases
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DNA Primers
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Endoribonucleases / genetics
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Endoribonucleases / metabolism*
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Gene Expression Regulation, Enzymologic
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Genomic Imprinting*
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Genotype
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Introns
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Mice
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Mice, Knockout
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Polymerase Chain Reaction
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Polymorphism, Genetic
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RNA Helicases / genetics*
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RNA Helicases / metabolism
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Ribonuclease III
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TRPC Cation Channels / genetics
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TRPC Cation Channels / metabolism*
Substances
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Caenorhabditis elegans Proteins
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DNA Primers
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TRPC Cation Channels
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Endoribonucleases
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Dicer1 protein, mouse
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Ribonuclease III
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DEAD-box RNA Helicases
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RNA Helicases