Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase

Toru Tanaka; Dai Nishimura; Ray-Chang Wu; Mutsuki Amano; Tatsuya Iso; Larry Kedes; Hiroshi Nishida; Kozo Kaibuchi; Yasuo Hamamori

doi:10.1074/jbc.M510954200

Nuclear Rho kinase, ROCK2, targets p300 acetyltransferase

J Biol Chem. 2006 Jun 2;281(22):15320-9. doi: 10.1074/jbc.M510954200. Epub 2006 Mar 30.

Authors

Toru Tanaka¹, Dai Nishimura, Ray-Chang Wu, Mutsuki Amano, Tatsuya Iso, Larry Kedes, Hiroshi Nishida, Kozo Kaibuchi, Yasuo Hamamori

Affiliation

¹ Department of Medicine and Center for Cardiovascular Development, Baylor College of Medicine, Houston, TX 77030, USA.

PMID: 16574662
DOI: 10.1074/jbc.M510954200

Abstract

Rho-associated coiled-coil protein kinase (ROCK) is an effector for the small GTPase Rho and plays a pivotal role in diverse cellular activities, including cell adhesion, cytokinesis, and gene expression, primarily through an alteration of actin cytoskeleton dynamics. Here, we show that ROCK2 is localized in the nucleus and associates with p300 acetyltransferase both in vitro and in cells. Nuclear ROCK2 is present in a large protein complex and partially cofractionates with p300 by gel filtration analysis. By immunofluorescence, ROCK2 partially colocalizes with p300 in distinct insoluble nuclear structures. ROCK2 phosphorylates p300 in vitro, and nuclear-restricted expression of constitutively active ROCK2 induces p300 phosphorylation in cells. p300 acetyltransferase activity is dependent on its phosphorylation status in cells, and p300 phosphorylation by ROCK2 results in an increase in its acetyltransferase activity in vitro. These observations suggest that nucleus-localized ROCK2 targets p300 for phosphorylation to regulate its acetyltransferase activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding Sites
Cattle
Cell Cycle Proteins / chemistry
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Nucleus / enzymology
Histone Acetyltransferases / chemistry
Histone Acetyltransferases / genetics
Histone Acetyltransferases / metabolism*
Humans
In Vitro Techniques
Intracellular Signaling Peptides and Proteins
Multiprotein Complexes
Peptide Mapping
Phosphorylation
Protein Serine-Threonine Kinases / chemistry
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Transcription Factors / chemistry
Transcription Factors / genetics
Transcription Factors / metabolism*
Two-Hybrid System Techniques
p300-CBP Transcription Factors
rho-Associated Kinases

Substances

Cell Cycle Proteins
Intracellular Signaling Peptides and Proteins
Multiprotein Complexes
Recombinant Fusion Proteins
Transcription Factors
Histone Acetyltransferases
p300-CBP Transcription Factors
p300-CBP-associated factor
Protein Serine-Threonine Kinases
ROCK2 protein, human
rho-Associated Kinases