Abstract
CUTL1, also known as CDP (CCAAT Displacement Protein), Cut, or Cux-1, is a homeodomain transcription factor known to play an essential role in development and cell cycle progression. Previously, we identified CUTL1 as modulator of cell motility and invasiveness. Here we report that protein kinase A (PKA), known to inhibit tumor progression in various tumor types, directly phosphorylates CUTL1 at serine 1215 in NIH3T3 fibroblasts. The PKA-induced phosphorylation results in decreased DNA binding affinity of CUTL1 and diminished CUTL1-mediated cell cycle progression and cell motility. Furthermore, the expression of several CUTL1 target genes involved in proliferation and migration, such as DNA polymerase A and DKK2, was modulated by PKA-induced phosphorylation. These data identify CUTL1 as a novel target of PKA through which this protein kinase can modulate tumor cell motility and tumor progression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Cell Cycle / physiology
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Cell Movement / physiology*
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Cell Proliferation
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Cyclic AMP / metabolism
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Cyclic AMP / pharmacology
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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In Vitro Techniques
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Mice
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NIH 3T3 Cells
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Phosphorylation
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RNA / genetics
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Serine / chemistry
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Transcription Factors
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Transcription, Genetic / drug effects
Substances
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CUX1 protein, human
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Cux1 protein, mouse
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Homeodomain Proteins
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Nuclear Proteins
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Recombinant Proteins
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Repressor Proteins
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Transcription Factors
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Serine
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RNA
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Cyclic AMP
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Cyclic AMP-Dependent Protein Kinases