Expression cloning and characterization of the TGF-beta type III receptor

Cell. 1991 Nov 15;67(4):797-805. doi: 10.1016/0092-8674(91)90074-9.

Abstract

The rat TGF-beta type III receptor cDNA has been cloned by overexpression in COS cells. The encoded receptor is an 853 amino acid protein with a large N-terminal extracellular domain containing at least one site for glycosaminoglycan addition, a single hydrophobic transmembrane domain, and a 41 amino acid cytoplasmic tail with no obvious signaling motif. Introduction of the cDNA into COS cells and L6 myoblasts induces expression of a heterogenously glycosylated 280-330 kd protein characteristic of the type III receptor that binds TGF-beta 1 specifically. In L6 myoblasts lacking the endogenous type III receptor, expression of the recombinant receptor leads to an increase in the amount of ligand bound and cross-linked to surface type II TGF-beta receptors. This indicates that the type III receptor may regulate the ligand-binding ability or surface expression of the type II receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology
  • Membrane Proteins / genetics*
  • Molecular Sequence Data
  • Proteoglycans / genetics*
  • Proteoglycans / immunology
  • RNA, Messenger / genetics
  • Rats
  • Receptors, Cell Surface / classification
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / immunology
  • Receptors, Transforming Growth Factor beta
  • Transfection
  • Transforming Growth Factor beta / metabolism*

Substances

  • Membrane Glycoproteins
  • Membrane Proteins
  • Proteoglycans
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta
  • betaglycan
  • DNA

Associated data

  • GENBANK/M80784
  • GENBANK/S56945
  • GENBANK/S60911
  • GENBANK/S60914
  • GENBANK/S60917
  • GENBANK/S60919
  • GENBANK/S60920
  • GENBANK/S60969
  • GENBANK/S61522
  • GENBANK/S61524
  • GENBANK/S65574