High-resolution physical mapping and construction of a porcine contig spanning the intramuscular fat content QTL

Anim Genet. 2006 Apr;37(2):113-20. doi: 10.1111/j.1365-2052.2005.01397.x.

Abstract

We previously mapped a locus for porcine intramuscular fat content (IMF) by linkage analysis to a 17.1-cM chromosome interval on Sus scrofa chromosome 7 (SSC7) flanked by microsatellite markers SW1083 and SW581. In this study, we identified 34 microsatellite markers and 14 STSs from the 17.1-cM IMF quantitative trait loci (QTL) region corresponding to HSA14q and aligned those loci using the INRA-University of Minnesota porcine radiation hybrid (IMpRH) panel. We then constructed a 5.2-Mb porcine bacterial artificial chromosome (BAC) contig of this region that was aligned using the RH panel. Finally, the IMF QTL was fine-mapped to 12.6 cM between SJ169 and MM70 at the 0.1% chromosome-wise significance level by genotyping the previously studied F2 resource family with 17 additional microsatellites. We also demonstrated that the SJ169-MM70 interval spans approximately 3.0 Mb and contains at least 12 genes: GALC, GPR65, KCNK10, SPATA7, PTPN21, FLJ11806, EML5, TTC8, CHES1, CAP2P1, CHORDC2P and C14orf143.

MeSH terms

  • Adipose Tissue / anatomy & histology
  • Adiposity / genetics*
  • Animals
  • Breeding
  • Chromosomes, Artificial, Bacterial
  • Contig Mapping*
  • Male
  • Meat / standards
  • Microsatellite Repeats
  • Quantitative Trait Loci*
  • Radiation Hybrid Mapping*
  • Swine / anatomy & histology
  • Swine / genetics*