Abstract
A full-length cDNA clone encoding the human gastric H,K-ATPase (EC 3.6.1.36)beta-subunit was isolated from a human gastric mucosal lambda gt10 library using oligonucleotide probes which were based on the cDNA sequence from rat and rabbit H,K-ATPase beta-subunits. The insert was 1407 bp in length and encoded a polypeptide of 291 amino acids with a MW = 33,367 Da. It exhibited 84.2%, 85.6% and 81.3% identity to the H,K-ATPase beta-subunits of rabbit, pig and rat, respectively.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics*
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Adenosine Triphosphatases / isolation & purification
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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DNA / genetics*
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Gastric Mucosa / enzymology*
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H(+)-K(+)-Exchanging ATPase
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Humans
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Molecular Sequence Data
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Molecular Weight
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Oligonucleotide Probes
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Rabbits
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Rats
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Sequence Homology, Nucleic Acid
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Swine
Substances
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Oligonucleotide Probes
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DNA
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Adenosine Triphosphatases
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H(+)-K(+)-Exchanging ATPase