Specific endonucleolytic cleavage of IGF-II mRNAs

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1509-16. doi: 10.1016/0006-291x(91)91743-v.

Abstract

Expression of the human insulin-like growth factor II (IGF-II) gene gives rise to a family of mRNAs which are expressed in a tissue- and developmental-specific manner. Recently, an IGF-II RNA species of 1.8 kb was detected which consists of the 3' terminal untranslated region of the last IGF-II exon. We have investigated the mechanism of formation of this RNA employing IGF-II minigenes. Analysis of the expression of these genes has revealed that the 1.8-kb RNA does not result from transcription activation, but arises by specific cleavage of IGF-II transcripts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Calorimetry
  • Cell Line
  • Cloning, Molecular
  • Endonucleases / metabolism*
  • Exons
  • Genes
  • Humans
  • Insulin / genetics
  • Insulin-Like Growth Factor II / genetics*
  • Mice
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plasmids
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • Rats
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Insulin
  • RNA, Messenger
  • Insulin-Like Growth Factor II
  • Endonucleases