Abstract
This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1(a) gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Decontamination / methods*
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Deoxyribonucleases / metabolism
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Equipment Contamination*
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Polymerase Chain Reaction / methods*
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Reagent Kits, Diagnostic*
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Taq Polymerase / genetics*
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Taq Polymerase / isolation & purification
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beta-Lactamases / genetics*
Substances
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Reagent Kits, Diagnostic
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Taq Polymerase
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Deoxyribonucleases
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beta-Lactamases
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beta-lactamase TEM-1