Supramolecular complexes mediate selenocysteine incorporation in vivo

Mol Cell Biol. 2006 Mar;26(6):2337-46. doi: 10.1128/MCB.26.6.2337-2346.2006.

Abstract

Selenocysteine incorporation in eukaryotes occurs cotranslationally at UGA codons via the interactions of RNA-protein complexes, one comprised of selenocysteyl (Sec)-tRNA([Ser]Sec) and its specific elongation factor, EFsec, and another consisting of the SECIS element and SECIS binding protein, SBP2. Other factors implicated in this pathway include two selenophosphate synthetases, SPS1 and SPS2, ribosomal protein L30, and two factors identified as binding tRNA([Ser]Sec), termed soluble liver antigen/liver protein (SLA/LP) and SECp43. We report that SLA/LP and SPS1 interact in vitro and in vivo and that SECp43 cotransfection increases this interaction and redistributes all three proteins to a predominantly nuclear localization. We further show that SECp43 interacts with the selenocysteyl-tRNA([Ser]Sec)-EFsec complex in vitro, and SECp43 coexpression promotes interaction between EFsec and SBP2 in vivo. Additionally, SECp43 increases selenocysteine incorporation and selenoprotein mRNA levels, the latter presumably due to circumvention of nonsense-mediated decay. Thus, SECp43 emerges as a key player in orchestrating the interactions and localization of the other factors involved in selenoprotein biosynthesis. Finally, our studies delineating the multiple, coordinated protein-nucleic acid interactions between SECp43 and the previously described selenoprotein cotranslational factors resulted in a model of selenocysteine biosynthesis and incorporation dependent upon both cytoplasmic and nuclear supramolecular complexes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Cell Nucleus / metabolism
  • Cells, Cultured
  • Codon, Terminator
  • Cytoplasm / metabolism
  • Humans
  • Multiprotein Complexes / metabolism*
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism
  • Phosphotransferases / genetics
  • Phosphotransferases / metabolism
  • RNA, Messenger / metabolism
  • RNA, Transfer, Ser / genetics
  • RNA, Transfer, Ser / metabolism
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Selenocysteine / metabolism*
  • Selenoproteins / biosynthesis
  • Selenoproteins / metabolism

Substances

  • Autoantigens
  • Codon, Terminator
  • EFsec protein, mouse
  • Multiprotein Complexes
  • Peptide Elongation Factors
  • RNA, Messenger
  • RNA, Transfer, Ser
  • RNA-Binding Proteins
  • SECISBP2 protein, human
  • Selenoproteins
  • Trnau1ap protein, rat
  • liver antigen LA-1
  • Selenocysteine
  • Phosphotransferases
  • selenophosphate synthetase