Capucin: a novel striatal marker down-regulated in rodent models of Huntington disease

Genomics. 2006 Feb;87(2):200-7. doi: 10.1016/j.ygeno.2005.10.009. Epub 2005 Dec 15.

Abstract

In an initial study, we compared quantitative transcriptome data across mouse brain territories using the serial analysis of gene expression method. Among the novel regional markers that we discovered, we focused on a striatum-enriched transcript with no available experimental cDNA sequence. Here, we report its cloning, gene structure, and detailed distribution in mouse brain. Quantitative RT-PCR and in situ hybridization demonstrated predominant expression in dorsolateral striatum. We therefore named it capucin for caudate-and putamen-enriched sequence. Mouse capucin is a 237-amino-acid protein, without any registered ortholog in mammalian species. It contains no recognizable motif other than two predicted carboxy-terminal transmembrane domains. When expressed in fusion with a fluorescent protein, it localized to the Golgi apparatus in two mammalian cell lines. Interestingly, we observed a significant down-regulation of capucin mRNA levels in two rodent models of Huntington disease, indicating a possible contribution to the pathogenesis of this disorder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biomarkers
  • Cloning, Molecular
  • Corpus Striatum / metabolism*
  • DNA, Complementary
  • Disease Models, Animal*
  • Down-Regulation*
  • Humans
  • Huntington Disease / genetics*
  • In Situ Hybridization
  • Male
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Subcellular Fractions / metabolism

Substances

  • Biomarkers
  • DNA, Complementary
  • Membrane Proteins
  • Syndig1l protein, mouse

Associated data

  • GENBANK/AY993932
  • GENBANK/AY993933
  • GENBANK/AY993934