Abstract
The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus Phages / genetics*
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Blotting, Southern
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DNA / genetics
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DNA, Circular / genetics
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DNA-Directed DNA Polymerase / chemistry
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DNA-Directed DNA Polymerase / genetics*
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Ethidium / pharmacology
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Genetic Techniques*
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Green Fluorescent Proteins / metabolism
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Nucleic Acid Amplification Techniques / methods*
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Plasmids / genetics*
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Plasmids / metabolism
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Software
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Templates, Genetic
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Time Factors
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Ultraviolet Rays
Substances
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DNA, Circular
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Green Fluorescent Proteins
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DNA
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DNA-Directed DNA Polymerase
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Ethidium