Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA

Biomol Eng. 2005 Oct;22(4):129-32. doi: 10.1016/j.bioeng.2005.05.001.

Abstract

The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus Phages / genetics*
  • Blotting, Southern
  • DNA / genetics
  • DNA, Circular / genetics
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / genetics*
  • Ethidium / pharmacology
  • Genetic Techniques*
  • Green Fluorescent Proteins / metabolism
  • Nucleic Acid Amplification Techniques / methods*
  • Plasmids / genetics*
  • Plasmids / metabolism
  • Software
  • Templates, Genetic
  • Time Factors
  • Ultraviolet Rays

Substances

  • DNA, Circular
  • Green Fluorescent Proteins
  • DNA
  • DNA-Directed DNA Polymerase
  • Ethidium