Abstract
Immunotherapy with tumor-specific peptide-loaded dendritic cells represents a promising therapeutic approach for patients with multifocal primary or early relapsed Ewing family tumors (EFT). The authors therefore screened a peptide library derived from the fusion region of the EFT-specific chimeric transcription factor EWS-FLI1 for immunogenic peptides. T-cell priming with 10 peptides was evaluated using IFN(gamma) video-assisted automated enzyme-linked immunospot technique. The authors report the identification of the first EFT-specific immunogenic T-cell epitope so far. Its identification will lead to a better understanding of EFT immunology and may improve DC-based immunotherapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigen Presentation
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Dendritic Cells / immunology
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Epitopes, T-Lymphocyte*
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HLA-DR Antigens / immunology*
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Humans
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Immunotherapy, Adoptive
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Interferon-gamma / analysis
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Oncogene Proteins, Fusion / immunology*
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Peptide Library
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Peptides / immunology
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Proto-Oncogene Protein c-fli-1
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RNA-Binding Protein EWS
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Sarcoma, Ewing
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T-Cell Antigen Receptor Specificity
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T-Lymphocytes, Helper-Inducer / immunology*
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Transcription Factors / immunology*
Substances
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EWS-FLI fusion protein
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Epitopes, T-Lymphocyte
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HLA-DR Antigens
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Oncogene Proteins, Fusion
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Peptide Library
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Peptides
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Proto-Oncogene Protein c-fli-1
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RNA-Binding Protein EWS
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Transcription Factors
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Interferon-gamma