Fluorometric assay for N-acetylprocainamide

Clin Chem. 1975 Dec;21(13):1899-902.

Abstract

We describe a simple, rapid fluorometric assay for separate quantitative analysis of procainamide and N-acetylprocainamide in mixtures. The effective lenear range (fluorescence vs. concentration) in serum is 0.1 to 10.0 mg/liter, regardless of the ratio (by weight) of the two drugs from 1:10 to 10:1. Analytical recoveries by the extraction method used were 100.0 +/- 3.0% and 98.0 +/- 4.0%, respectively. For determination of either compound, the maximum coefficient of variation was 10%.

MeSH terms

  • Humans
  • Hydrogen-Ion Concentration
  • Procainamide / analogs & derivatives*
  • Procainamide / blood
  • Spectrometry, Fluorescence

Substances

  • Procainamide