Abstract
Technologies to assess the molecular targets of biomolecules in living cells are lacking. We have developed a technology called magnetism-based interaction capture (MAGIC) that identifies molecular targets on the basis of induced movement of superparamagnetic nanoparticles inside living cells. Efficient intracellular uptake of superparamagnetic nanoparticles (coated with a small molecule of interest) was mediated by a transducible fusogenic peptide. These nanoprobes captured the small molecule's labeled target protein and were translocated in a direction specified by the magnetic field. Use of MAGIC in genome-wide expression screening identified multiple protein targets of a drug. MAGIC was also used to monitor signal-dependent modification and multiple interactions of proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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Caspase 3
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Caspases / metabolism
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Cell Line
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Cell Physiological Phenomena*
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Cell Survival
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Endocytosis
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Fluorescein-5-isothiocyanate
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Fluorescent Dyes
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HeLa Cells
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Humans
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I-kappa B Proteins / metabolism
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Magnetics*
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Microscopy, Confocal
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Molecular Probe Techniques*
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Molecular Probes*
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NF-KappaB Inhibitor alpha
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NF-kappa B / metabolism
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Nanostructures*
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Oligopeptides / metabolism
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Phosphorylation
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Protein Binding
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Proteins / metabolism*
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Quantum Dots
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Recombinant Fusion Proteins / metabolism
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Signal Transduction
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Streptavidin
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Tacrolimus / metabolism
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Transcription Factor RelA
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Tumor Necrosis Factor-alpha / pharmacology
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beta-Transducin Repeat-Containing Proteins / metabolism
Substances
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Fluorescent Dyes
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I-kappa B Proteins
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Molecular Probes
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NF-kappa B
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NFKBIA protein, human
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Oligopeptides
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Proteins
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Recombinant Fusion Proteins
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Transcription Factor RelA
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Tumor Necrosis Factor-alpha
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aspartyl-glutamyl-valyl-aspartal
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beta-Transducin Repeat-Containing Proteins
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NF-KappaB Inhibitor alpha
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Streptavidin
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CASP3 protein, human
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Caspase 3
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Caspases
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Fluorescein-5-isothiocyanate
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Tacrolimus