Abstract
A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bacterial DNA replication and cell viability, as well as the PolI-dependent replication of the plasmid carrying dnaE.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA Polymerase I / genetics
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DNA Polymerase I / metabolism*
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DNA Polymerase III / biosynthesis*
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DNA Polymerase III / genetics
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DNA Replication / genetics*
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DNA, Bacterial / genetics
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Mutation / genetics
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Plasmids / genetics
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Rec A Recombinases / genetics
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Temperature
Substances
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DNA, Bacterial
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DNA polymerase III, alpha subunit
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Rec A Recombinases
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DNA Polymerase I
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DNA Polymerase III