Nucleotide sequence of canine c-N-ras: codons 1 to 71

Am J Vet Res. 1992 Apr;53(4):600-3.

Abstract

The c-N-ras gene has been implicated often in the genesis and/or progression of human leukemias. To our knowledge, the sequence of this gene in the dog has not been reported. Using a system of asymmetric reamplification of double-stranded polymerase chain reaction (PCR) products, we have sequenced normal canine c-N-ras mRNA from position -26 to +213, including codons 12, 13, and 61, which are the sites where oncogenic mutations are most commonly observed. The canine c-N-ras sequence has close homology with the human sequence in this area; there were only 6 observed base differences in nucleotide sequence and none resulted in a change of the encoded amino acid. The results of this study set the stage for directed searches for c-N-ras mutations in experimentally induced and naturally acquired neoplasms of the dog.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoradiography
  • Base Sequence
  • Codon / chemistry*
  • DNA / chemistry*
  • Dogs / genetics*
  • Genes, ras / genetics*
  • Molecular Sequence Data
  • Mutation
  • Polymerase Chain Reaction
  • RNA, Messenger / chemistry*
  • Sequence Homology, Nucleic Acid
  • Species Specificity

Substances

  • Codon
  • RNA, Messenger
  • DNA