Genomic organization, promoter characterization and roles of Sp1 and AP-2 in the basal transcription of mouse PDIP1 gene

FEBS Lett. 2005 Mar 14;579(7):1715-22. doi: 10.1016/j.febslet.2005.02.027.

Abstract

The mouse polymerase delta-interacting protein 1 gene, PDIP1, is mapped to chromosome 7F3 region, spans approximately 16.7kb, and is organized into six exons. The transcription start site (TSS) was determined to be G, corresponding to position of 162-bp upstream of the translation start codon. The promoter region was found to lack TATA box or CCAAT box, instead, a CpG island was detected surrounding TSS. The region from -162 to +114 is required for basal transcriptional regulation of mouse PDIP1 gene, contains two AP-2 and two Sp1 binding sites. The Sp1 site upstream of TSS activates, while the other Sp1 site and two AP-2 sites suppress the transcription activity of mouse PDIP1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Carrier Proteins / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Down-Regulation
  • Exons / genetics
  • Gene Components
  • Genome
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics*
  • Sp1 Transcription Factor / genetics
  • Sp1 Transcription Factor / physiology*
  • Transcription Factor AP-2
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transcription Initiation Site
  • Transcription, Genetic
  • Ubiquitin-Protein Ligase Complexes
  • Up-Regulation

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Sp1 Transcription Factor
  • Transcription Factor AP-2
  • Transcription Factors
  • PDIP1 protein, mouse
  • Ubiquitin-Protein Ligase Complexes