Abstract
Dimethylsulfoxide (DMSO) has been known to differentiate HL60 cells into neutrophil like cells. Here, we provide an evidence for the involvement of tumor suppressor PTEN, an antagonist of phosphatidylinositol 3-kinase (PI3K) in the DMSO-induced differentiation of HL60 cells. DMSO upregulated PTEN with unaffecting the expression of PI3K. The upregulation of PTEN by DMSO lead to the decrease of Akt phosphorylation, a downstream of PI3K. The DMSO-induced upregulation of PTEN might be mediated by NF-kappaB activation, which was evidenced by the blockage of DMSO-induced PTEN upregulation with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers
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Dimethyl Sulfoxide / pharmacology*
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Gene Expression Regulation, Neoplastic / drug effects*
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Genes, Tumor Suppressor* / drug effects
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HL-60 Cells
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Humans
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Kinetics
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NF-kappa B / metabolism*
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PTEN Phosphohydrolase
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Phosphoinositide-3 Kinase Inhibitors
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Phosphoric Monoester Hydrolases / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Suppressor Proteins / genetics*
Substances
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DNA Primers
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NF-kappa B
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Phosphoinositide-3 Kinase Inhibitors
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Tumor Suppressor Proteins
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Phosphoric Monoester Hydrolases
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PTEN Phosphohydrolase
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PTEN protein, human
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Dimethyl Sulfoxide