Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis

Genome Biol. 2005;6(1):R6. doi: 10.1186/gb-2004-6-1-r6. Epub 2004 Dec 23.

Abstract

A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential of a large fraction of B-cell specific and housekeeping genes whose function can be analyzed by disruption in the chicken DT40 B-cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Avian Proteins / chemistry
  • Avian Proteins / genetics*
  • Avian Proteins / metabolism*
  • Base Composition / genetics
  • Bursa of Fabricius / cytology*
  • Bursa of Fabricius / metabolism
  • Cell Line
  • Chickens / genetics*
  • Cloning, Molecular
  • Codon, Initiator / genetics
  • Computational Biology*
  • Conserved Sequence
  • DNA, Complementary / genetics*
  • Databases, Genetic
  • Lymphocytes / metabolism*
  • Organ Specificity
  • Protein Structure, Tertiary
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology
  • Transcription, Genetic / genetics

Substances

  • Avian Proteins
  • Codon, Initiator
  • DNA, Complementary
  • RNA, Messenger