A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses

Neil Hall; Marianna Karras; J Dale Raine; Jane M Carlton; Taco W A Kooij; Matthew Berriman; Laurence Florens; Christoph S Janssen; Arnab Pain; Georges K Christophides; Keith James; Kim Rutherford; Barbara Harris; David Harris; Carol Churcher; Michael A Quail; Doug Ormond; Jon Doggett; Holly E Trueman; Jacqui Mendoza; Shelby L Bidwell; Marie-Adele Rajandream; Daniel J Carucci; John R Yates 3rd; Fotis C Kafatos; Chris J Janse; Bart Barrell; C Michael R Turner; Andrew P Waters; Robert E Sinden

doi:10.1126/science.1103717

A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses

Science. 2005 Jan 7;307(5706):82-6. doi: 10.1126/science.1103717.

Affiliation

¹ Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK. nhall@tigr.org

PMID: 15637271
DOI: 10.1126/science.1103717

Abstract

Plasmodium berghei and Plasmodium chabaudi are widely used model malaria species. Comparison of their genomes, integrated with proteomic and microarray data, with the genomes of Plasmodium falciparum and Plasmodium yoelii revealed a conserved core of 4500 Plasmodium genes in the central regions of the 14 chromosomes and highlighted genes evolving rapidly because of stage-specific selective pressures. Four strategies for gene expression are apparent during the parasites' life cycle: (i) housekeeping; (ii) host-related; (iii) strategy-specific related to invasion, asexual replication, and sexual development; and (iv) stage-specific. We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

3' Untranslated Regions
Animals
Anopheles / parasitology
Computational Biology
Evolution, Molecular
Gene Expression Profiling
Gene Silencing
Genes, Protozoan
Genome, Protozoan*
Life Cycle Stages*
Malaria / parasitology
Oligonucleotide Array Sequence Analysis
Plasmodium / genetics*
Plasmodium / growth & development*
Plasmodium / metabolism
Plasmodium berghei / genetics
Plasmodium berghei / growth & development
Plasmodium berghei / metabolism
Plasmodium chabaudi / genetics
Plasmodium chabaudi / growth & development
Plasmodium chabaudi / metabolism
Plasmodium falciparum / genetics
Plasmodium falciparum / growth & development
Plasmodium falciparum / metabolism
Plasmodium yoelii / genetics
Plasmodium yoelii / growth & development
Plasmodium yoelii / metabolism
Proteome / analysis*
Proteomics
Protozoan Proteins / analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Protozoan / genetics
RNA, Protozoan / metabolism
Selection, Genetic
Transcription, Genetic

Substances

3' Untranslated Regions
Proteome
Protozoan Proteins
RNA, Messenger
RNA, Protozoan