Novel human BTB/POZ domain-containing zinc finger protein ZNF295 is directly associated with ZFP161

Biochem Biophys Res Commun. 2005 Feb 11;327(2):615-27. doi: 10.1016/j.bbrc.2004.12.048.

Abstract

Human ZNF295 protein harbors a BTB/POZ domain and multiple krüppel (C(2)H(2)) type zinc finger domains, and thus belongs to a family of POK (POZ and krüppel) transcription factor. We have identified many transcript variants generated by the alternative splicing in 5' non-coding exons, an intra-exonic splicing in a coding region, and the use of three polyadenylation signals in the 3' UTR. The intra-exonic splicing removes 603-bp coding sequence, and thus ZNF295 gene produces two protein isoforms: ZNF295L with 1066 amino acid residues and ZNF295S with 865 amino acid residues, containing 9 and 5 zinc finger domains, respectively. ZNF295 is ubiquitously expressed in human fetal and adult tissues. Analysis of transcription activity of ZNF295 using various promoter-reporters demonstrated that ZNF295 acts as a transcription repressor, and contains two separate regions for repression activity: the BTB/POZ domain and the central region between BTB/POZ and ZF domains. Both ZNF295L and ZNF295S could interact not only with themselves and each other, but also with another POK protein ZFP161 known to function as a transcription repressor and an activator. We postulated that ZNF295 may be involved in the bi-directional control of gene expression in concert with ZFP161.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Exons / genetics
  • Gene Expression Profiling
  • Humans
  • Introns / genetics
  • Kruppel-Like Transcription Factors
  • Molecular Sequence Data
  • Protein Binding
  • Protein Structure, Tertiary
  • Protein Transport
  • Repressor Proteins / chemistry
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Sequence Alignment
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic / genetics
  • Zinc Fingers*

Substances

  • DNA-Binding Proteins
  • Kruppel-Like Transcription Factors
  • Repressor Proteins
  • Transcription Factors
  • ZBTB14 protein, human
  • ZBTB21 protein, human

Associated data

  • GENBANK/AB041014
  • GENBANK/AB041015
  • GENBANK/AB190372
  • GENBANK/AB190373
  • GENBANK/AB190374