Regulation of procaspase-2 by glucocorticoid modulatory element-binding protein 1 through the interaction with caspase recruitment domain

Biochem Biophys Res Commun. 2004 Dec 24;325(4):1246-51. doi: 10.1016/j.bbrc.2004.10.145.

Abstract

Caspases are the primary executioners of apoptosis. Although procaspases believe to exist as inactive forms in cells, the detailed regulatory system remains unclear. Here we show that glucocorticoid modulatory element-binding protein 1 (GMEB1) is capable of binding to the prodomain of caspase-2. We found that this molecule inhibits the autoproteolytic activation of procaspase-2 by oligomerization on a chemical compound-dependent system. These findings indicated that GMEB1 might be an endogenous inhibitory protein that selectively interacts with prodomain of caspase-2 to disrupt the autoactivation.

MeSH terms

  • Apoptosis
  • Caspase 2
  • Caspases / genetics
  • Caspases / metabolism*
  • Cell Line
  • DNA-Binding Proteins / metabolism*
  • Enzyme Activation
  • Gene Expression Regulation
  • HeLa Cells
  • Humans
  • Kidney / metabolism*
  • Nucleoside-Phosphate Kinase / metabolism*
  • Protein Structure, Tertiary
  • Recombinant Proteins / metabolism
  • Transcription Factors / metabolism*

Substances

  • DNA-Binding Proteins
  • Recombinant Proteins
  • Transcription Factors
  • p96 subunit, parvovirus initiation factor
  • Nucleoside-Phosphate Kinase
  • Caspase 2
  • Caspases