Abstract
Lack of regular cell-cell interaction is one major cause for neoplastic growth and metastasis. In head and neck squamous cell carcinomas a 10-fold down-regulation of connexin31.1 (GJB5) as well as mutations in the TGF-beta-receptor-II were reported. We performed mutation screenings in GJB5 and the TGF-beta-receptor-II poly(10)adenine hot spot employing larynx cancer samples of 10 patients. Variable length of the TGF-beta-receptor-II adenine homopolymer in controls and tumours indicate a high slippage error rate of the DNA polymerases rendering mutational analyses inconsistent. Lack of GJB5 mutations in the entire tumour collection suggests that this gene is not primarily involved in laryngeal tumorigenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Carcinoma, Squamous Cell / genetics*
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Carcinoma, Squamous Cell / pathology
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Case-Control Studies
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Cell Communication
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Connexins / biosynthesis*
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DNA Mutational Analysis
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DNA-Directed DNA Polymerase / pharmacology
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Down-Regulation
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Female
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Humans
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Laryngeal Neoplasms / genetics*
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Laryngeal Neoplasms / pathology
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Loss of Heterozygosity
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Male
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Middle Aged
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Polymerase Chain Reaction
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type II
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Receptors, Transforming Growth Factor beta / genetics*
Substances
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Connexins
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Receptors, Transforming Growth Factor beta
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connexin 31.1
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Protein Serine-Threonine Kinases
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Receptor, Transforming Growth Factor-beta Type II
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DNA-Directed DNA Polymerase