The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner

Brian J Wilson; Gaynor J Bates; Samantha M Nicol; David J Gregory; Neil D Perkins; Frances V Fuller-Pace

doi:10.1186/1471-2199-5-11

The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner

BMC Mol Biol. 2004 Aug 6:5:11. doi: 10.1186/1471-2199-5-11.

Authors

Brian J Wilson¹, Gaynor J Bates, Samantha M Nicol, David J Gregory, Neil D Perkins, Frances V Fuller-Pace

Affiliation

¹ Department of Molecular and Cellular Pathology, Ninewells Medical School, University of Dundee, DD1 9SY, UK. brian.wilson@molonc.mcgill.ca

Abstract

Background: p68 (Ddx5) and p72 (Ddx17) are highly related members of the DEAD box family and are established RNA helicases. They have been implicated in growth regulation and have been shown to be involved in both pre-mRNA and pre-rRNA processing. More recently, however, these proteins have been reported to act as transcriptional co-activators for estrogen-receptor alpha (ER alpha). Furthermore these proteins were shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme. Taken together these reports suggest a role for p68 and p72 in transcriptional activation.

Results: In this report we show that p68 and p72 can, in some contexts, act as transcriptional repressors. Targeting of p68 or p72 to constitutive promoters leads to repression of transcription; this repression is promoter-specific. Moreover both p68 and p72 associate with histone deacetylase 1 (HDAC1), a well-established transcriptional repression protein.

Conclusions: It is therefore clear that p68 and p72 are important transcriptional regulators, functioning as co-activators and/or co-repressors depending on the context of the promoter and the transcriptional complex in which they exist.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / pathology
Adenosine Triphosphatases / chemistry
Adenosine Triphosphatases / genetics
Adenosine Triphosphatases / physiology*
Adenoviridae / genetics
Bone Neoplasms / pathology
Breast Neoplasms / pathology
Cell Line
Cell Line, Tumor
Chromatography, Gel
DEAD-box RNA Helicases
Enhancer Elements, Genetic / genetics
Genes, Reporter
Herpes Simplex / genetics
Histone Deacetylase 1
Histone Deacetylases / physiology*
Humans
Kidney
Osteosarcoma / pathology
Peptide Fragments / chemistry
Peptide Fragments / physiology
Promoter Regions, Genetic / genetics*
Protein Interaction Mapping
Protein Kinases / chemistry
Protein Kinases / genetics
Protein Kinases / physiology*
Protein Structure, Tertiary
RNA Helicases / chemistry
RNA Helicases / genetics
RNA Helicases / physiology*
Recombinant Fusion Proteins / physiology
Repressor Proteins / chemistry
Repressor Proteins / genetics
Repressor Proteins / physiology*
Sequence Deletion
Simian virus 40 / genetics
Thymidine Kinase / genetics
Trans-Activators / chemistry
Trans-Activators / genetics
Trans-Activators / physiology*
Transcription, Genetic*
Transfection

Substances

Peptide Fragments
Recombinant Fusion Proteins
Repressor Proteins
Trans-Activators
Protein Kinases
Thymidine Kinase
HDAC1 protein, human
Histone Deacetylase 1
Histone Deacetylases
Adenosine Triphosphatases
DDX17 protein, human
Ddx5 protein, human
DEAD-box RNA Helicases
RNA Helicases