Regulation of STAT3 by mu-opioid receptors in human neuroblastoma SH-SY5Y cells

Jessie W F Yuen; Ivy Y W So; Angel Y F Kam; Yung H Wong

doi:10.1097/01.wnr.0000130433.90962.6e

Regulation of STAT3 by mu-opioid receptors in human neuroblastoma SH-SY5Y cells

Neuroreport. 2004 Jun 28;15(9):1431-5. doi: 10.1097/01.wnr.0000130433.90962.6e.

Authors

Jessie W F Yuen¹, Ivy Y W So, Angel Y F Kam, Yung H Wong

Affiliation

¹ Department of Biochemistry, the Molecular Neuroscience Center, and the Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

PMID: 15194868
DOI: 10.1097/01.wnr.0000130433.90962.6e

Abstract

Heptahelical opioid receptors are implicated in the transcriptional regulation of neuronal development. Here we demonstrated that activation of mu-opioid receptors in human neuroblastoma SH-SY5Y cells led to the activation of signal transducer and activator of transcription 3 (STAT3), a transcription factor central to the regulation of numerous biological processes. The mu-opioid-induced activation of STAT3 is sensitive to receptor was further shown to pertussis toxin treatment and required JAK and Src tyrosine kinases, but not phosphatidylinositol 3-kinase. This mu-opioid-induced response was mediated via the extracellular signal-regulated protein kinase in a Raf-1-independent manner. The present study provides a foundation to explore the importance of STAT3 signaling in the regulation of neuronal growth and differentiation by the mu-opioid receptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor / drug effects
Cell Line, Tumor / enzymology
DNA-Binding Proteins / metabolism*
GTP-Binding Protein alpha Subunits, Gi-Go / metabolism
Humans
JNK Mitogen-Activated Protein Kinases
MAP Kinase Kinase Kinase 1*
MAP Kinase Kinase Kinases / metabolism
MAP Kinase Signaling System / physiology*
Mitogen-Activated Protein Kinases / metabolism
Neuroblastoma*
Pertussis Toxin / pharmacology
Phosphatidylinositol 3-Kinases / metabolism
Proto-Oncogene Proteins c-raf / metabolism
Receptors, Opioid, mu / metabolism*
STAT3 Transcription Factor
Trans-Activators / metabolism*
Tyrosine / metabolism
src-Family Kinases / metabolism

Substances

DNA-Binding Proteins
Receptors, Opioid, mu
STAT3 Transcription Factor
STAT3 protein, human
Trans-Activators
Tyrosine
Pertussis Toxin
Phosphatidylinositol 3-Kinases
src-Family Kinases
Proto-Oncogene Proteins c-raf
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
MAP Kinase Kinase Kinase 1
MAP Kinase Kinase Kinases
MAP3K1 protein, human
GTP-Binding Protein alpha Subunits, Gi-Go