Abstract
The class VI POU domain family member known as Emb in the mouse (rat Brn5 or human mPOU/TCFbeta1) is present in vivo as a protein migrating at about 80 kDa on western blots, considerably larger than that predicted (about 42 kDa) from previously cloned coding sequences. By RT-PCR and 5' RACE strategies a full-length Emb sequence, Emb FL, is now identified. Shorter sequences encoding the -COOH terminal, and an -NH(2) terminal isoform, EmbN, were also isolated. Comparisons of Emb coding sequences between species, including the full-length zebra fish, POU(c), are presented, together with a compilation of the multiple transcripts produced by alternative splicing and the presence of different transcriptional start and stop sites, from the Emb gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Western
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Cell Line
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Cell Line, Tumor
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Genes / genetics
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Humans
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Molecular Sequence Data
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POU Domain Factors
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Protein Isoforms / genetics
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Rats
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic / genetics
Substances
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DNA, Complementary
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DNA-Binding Proteins
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POU Domain Factors
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Pou6f1 protein, mouse
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Pou6f1 protein, rat
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Protein Isoforms
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Transcription Factors
Associated data
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GENBANK/AY259046
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GENBANK/AY259047