Sp1 is involved in the transcriptional activation of p16(INK4) by p21(Waf1) in HeLa cells

Lixiang Xue; Junfeng Wu; Wenjie Zheng; Peichang Wang; Jun Li; Zongyu Zhang; Tanjun Tong

doi:10.1016/S0014-5793(04)00352-7

Sp1 is involved in the transcriptional activation of p16(INK4) by p21(Waf1) in HeLa cells

FEBS Lett. 2004 Apr 23;564(1-2):199-204. doi: 10.1016/S0014-5793(04)00352-7.

Authors

Lixiang Xue¹, Junfeng Wu, Wenjie Zheng, Peichang Wang, Jun Li, Zongyu Zhang, Tanjun Tong

Affiliation

¹ Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, 38 Xueyuan Road, Beijing 100083, PR China.

PMID: 15094066
DOI: 10.1016/S0014-5793(04)00352-7

Abstract

Both p16(INK4) and p21(Waf1) are very important negative regulators of the cell cycle. In this study we examined the effects of p21(Waf1) on the transcription of p16(INK4). We determined that p21(Waf1) can activate the transcription of p16(INK4), and that this effect is GC-box dependent. We also found that the transcription factor Sp1 plays a key role in this event. Upregulation of Sp1 contributes to the transcriptional activation and protein level of p16(INK4) mediated by p21(Waf1), and is a potential point of cooperation between the p16/pRb and p14 (ARF)/p53 tumor suppressor pathways.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclin-Dependent Kinase Inhibitor p16 / biosynthesis*
Cyclin-Dependent Kinase Inhibitor p16 / genetics
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / genetics*
Cyclins / physiology
HeLa Cells
Humans
Mutation
Promoter Regions, Genetic / genetics
Sp1 Transcription Factor / genetics*
Sp1 Transcription Factor / physiology
Transcriptional Activation*
Transfection
Tumor Suppressor Protein p14ARF
Up-Regulation

Substances

CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinase Inhibitor p21
Cyclins
Sp1 Transcription Factor
Tumor Suppressor Protein p14ARF