PRDM5 is silenced in human cancers and has growth suppressive activities

Oncogene. 2004 Jun 17;23(28):4903-10. doi: 10.1038/sj.onc.1207615.

Abstract

Several genes that contain the PR (PRDI-BF1 and RIZ) domain have been linked with human cancers. We describe here a new PR-domain-containing gene designated as PRDM5 (PFM2). A PRDM5 cDNA was isolated based on its homology to the PR domain of RIZ1 (PRDM2). The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Radiation hybrid analysis mapped PRDM5 to human chromosome 4q26, a region thought to harbor tumor suppressor genes for breast, ovarian, liver, lung, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. A recombinant adenovirus expressing PRDM5 caused G2/M arrest and apoptosis upon infection of tumor cells. These results suggest that inactivation of PRDM5 may play a role in carcinogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Apoptosis / genetics
  • Base Sequence
  • Breast
  • Breast Neoplasms
  • Carcinoma, Hepatocellular
  • Cell Cycle / genetics
  • Cell Division / genetics*
  • Cell Line, Tumor
  • DNA-Binding Proteins / genetics*
  • Dinucleoside Phosphates / chemistry
  • Female
  • Gene Silencing*
  • Humans
  • Liver
  • Liver Neoplasms
  • Molecular Sequence Data
  • Ovarian Neoplasms
  • Transcription Factors

Substances

  • DNA-Binding Proteins
  • Dinucleoside Phosphates
  • PRDM5 protein, human
  • Transcription Factors
  • cytidylyl-3'-5'-guanosine