Somatic cell mapping of the human cyclophilin B gene (PPIB) to chromosome 15

Cytogenet Cell Genet. 1992;60(3-4):219-21. doi: 10.1159/000133343.

Abstract

The polymerase chain reaction (PCR) technique was used to generate a unique probe complementary to the hydrophobic 5' end of the human cyclophilin B gene. This unique probe was hybridized to DNAs from human x hamster hybrid somatic cell lines retaining different combinations of human chromosomes. The gene was assigned to chromosome 15.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Isomerases / genetics*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Carrier Proteins / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 15*
  • Cyclosporins / genetics*
  • DNA Probes / genetics
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / genetics
  • Peptidylprolyl Isomerase
  • Polymerase Chain Reaction

Substances

  • Carrier Proteins
  • Cyclosporins
  • DNA Probes
  • Oligodeoxyribonucleotides
  • Amino Acid Isomerases
  • Peptidylprolyl Isomerase