Dehydrotrametenonic acid and dehydroeburiconic acid from Poria cocos and their inhibitory effects on eukaryotic DNA polymerase alpha and beta

Biosci Biotechnol Biochem. 2004 Feb;68(2):448-50. doi: 10.1271/bbb.68.448.

Abstract

A new lanostane-type triterpene acid, (20xi)-3-oxolanosta-7,9(11),24-trien-21-oic acid (1; dehydrotrametenonic acid), along with a known triterpene acid, dehydroeburiconic acid (2), were isolated from the epidermis of the sclerotia of Poria cocos. The structure of 1 was analyzed on the basis of spectroscopic methods. Compounds 1 and 2 inhibited calf DNA polymerase alpha and rat DNA polymerase beta, with the 50% inhibition values of 45.5 microM (1) and 40.8 microM (2), and 86.5 microM (1) and 30.0 microM (2), respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • DNA Polymerase I / antagonists & inhibitors*
  • DNA Polymerase beta / antagonists & inhibitors*
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Weight
  • Polyporales / chemistry*
  • Rats
  • Triterpenes / chemical synthesis*
  • Triterpenes / isolation & purification*
  • Triterpenes / pharmacology*

Substances

  • Enzyme Inhibitors
  • Triterpenes
  • dehydroeburiconic acid
  • dehydrotrametenonic acid
  • DNA Polymerase I
  • DNA Polymerase beta