Abstract
Neuronal calcium sensor (NCS) proteins including the subfamily of visinin-like-proteins (VILIPs) are involved in regulation of various signaling cascades. One molecular regulation mechanism is the calcium-myristoyl switch. VILIPs show a calcium-dependent membrane association in brain homogenates; however, differences in calcium-induced conformation changes and degree of membrane association are reported. Little is known about differences in the calcium-myristoyl switch in living cells leading to localization of VILIPs to distinct subcellular compartments. Therefore, we studied the calcium-dependent localization of green fluorescent protein (GFP)-tagged VILIP-3 in living cell lines and hippocampal neurons and compared it with that of GFP-VILIP-1. Interestingly, the observed fast and reversible calcium-myristoyl switch of VILIP-3-GFP and VILIP-1-GFP differed, e.g., in calcium-dependent translocation to Golgi membranes. Similarily, the calcium-dependent localization of endogenously expressed VILIP-3 and -1 in dendrites differed. Thus, VILIPs co-expressed in the same neuron show clear differences in calcium-dependent localization which may allow neurons a highly selective response to various calcium stimuli.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Calcium / metabolism
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Calcium Signaling / physiology*
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Calcium-Binding Proteins / metabolism*
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Cell Compartmentation / physiology
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Cell Membrane / metabolism
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Dendrites / metabolism
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Glutamate Decarboxylase / metabolism
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Golgi Apparatus / metabolism
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Green Fluorescent Proteins
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Hippocampus / cytology
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Hippocampus / metabolism*
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Luminescent Proteins
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Membrane Proteins / metabolism
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Mice
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Microtubule-Associated Proteins / metabolism
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Myristic Acid / metabolism
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Nerve Tissue Proteins / metabolism*
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Neurocalcin
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Neurons / cytology
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Neurons / metabolism*
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Potassium Chloride / pharmacology
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Protein Transport / physiology
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Qa-SNARE Proteins
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Rats
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Receptors, Calcium-Sensing / metabolism*
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Receptors, Glutamate / drug effects
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Receptors, Glutamate / metabolism
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Recombinant Fusion Proteins
Substances
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Calcium-Binding Proteins
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Hpcal1 protein, rat
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Luminescent Proteins
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Membrane Proteins
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Microtubule-Associated Proteins
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Nerve Tissue Proteins
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Neurocalcin
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Qa-SNARE Proteins
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Receptors, Calcium-Sensing
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Receptors, Glutamate
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Recombinant Fusion Proteins
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Vsnl1 protein, mouse
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Vsnl1 protein, rat
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Myristic Acid
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Green Fluorescent Proteins
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Potassium Chloride
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Glutamate Decarboxylase
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Calcium