Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2

Eugenia Pechkova; Giuseppe Zanotti; Claudio Nicolini

doi:10.1107/s0907444903018900

Three-dimensional atomic structure of a catalytic subunit mutant of human protein kinase CK2

Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2133-9. doi: 10.1107/s0907444903018900. Epub 2003 Nov 27.

Authors

Eugenia Pechkova¹, Giuseppe Zanotti, Claudio Nicolini

Affiliation

¹ Fondazione E.L.B.A., Rome, Italy.

PMID: 14646071
DOI: 10.1107/s0907444903018900

Abstract

The three-dimensional crystal structure of the triple-point mutant of the catalytic subunit of human protein kinase CK2alpha has been determined at 2.4 A resolution. Microcrystals of mutant CK2 catalytic subunit were obtained by a protein-crystallization method based on thin-film nanotechnology. These microcrystals (of about 20 micro m in diameter) were used for diffraction data collection by means of the microfocus beamline at the ESRF synchrotron. A comparison between the human protein kinase CK2alpha and the corresponding enzyme from a lower organism (Zea mays) is made.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Binding Sites
Casein Kinase II
Catalytic Domain
Crystallization / methods
Crystallography, X-Ray
Escherichia coli / genetics
Escherichia coli / metabolism
Humans
Models, Molecular
Molecular Sequence Data
Point Mutation
Protein Conformation
Protein Serine-Threonine Kinases / chemistry*
Protein Serine-Threonine Kinases / genetics
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Static Electricity
Zea mays / enzymology
Zea mays / genetics

Substances

Recombinant Proteins
Casein Kinase II
Protein Serine-Threonine Kinases