A new 9-lipoxygenase cDNA from developing rice see...[Plant Cell Physiol. 2003]

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1: Plant Cell Physiol. 2003 Nov;44(11):1168-75. Links

A new 9-lipoxygenase cDNA from developing rice seeds.

Mizuno K, Iida T, Takano A, Yokoyama M, Fujimura T.

Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. koumno@akita-pu.ac.jp

We isolated a novel C9 position specific lipoxygenase (r9-LOX1) cDNA from developing rice seeds. The enzymatic features of r9-LOX1 resembled those of rice LOX-L3 known to be contained in rice germ and to have C9-specific LOX activity. However, the expression level of the r9-LOX1 gene was higher in imbibed seeds rather than developing seeds. A homology search against the rice nucleotide database revealed the r9-LOX1 gene to be on rice chromosome 3 (accession number AC093017). The restriction enzyme map of the reported genomic sequence agreed with the result of the Southern blot analysis for the r9-LOX1. The enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid.

PMID: 14634153 [PubMed - indexed for MEDLINE]

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ReviewLipoxygenase gene disruption studies. Status and applications. [Adv Exp Med Biol. 1999]

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A new 9-lipoxygenase cDNA from developing rice seeds.

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