EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD

Gene. 2003 Oct 30:318:35-43. doi: 10.1016/j.gene.2003.06.001.

Abstract

Skeletal muscle differentiation has been shown to be dependent on the expression of Rb and p300. We recently cloned a novel inhibitor of muscle differentiation called EID-1, which interacted with both of these factors. In a database search for related molecules, we have cloned and characterized a new EID-1 family member, EID-2. This 28-kDa protein encodes a 236-amino-acid protein with significant similarity to EID-1 in its C-terminus. EID-2 displays developmentally regulated expression with high levels in adult heart and brain. Overexpression of EID-2 inhibits muscle-specific gene expression through inhibition of MyoD-dependent transcription. This inhibitory effect on gene expression can be explained by EID-2's ability to associate with and inhibit the acetyltransferase activity of p300. These data suggest that EID-1 and -2 represent a novel family of proteins that negatively regulate differentiation through a p300-dependent mechanism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / metabolism
  • Actins / genetics
  • Actins / metabolism
  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Line
  • Cell Line, Tumor
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Female
  • Gene Expression
  • Histone Acetyltransferases
  • Humans
  • Inhibitor of Differentiation Protein 2
  • Intracellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • Molecular Weight
  • Muscle, Skeletal / cytology
  • Muscle, Skeletal / metabolism
  • MyoD Protein / genetics
  • MyoD Protein / metabolism*
  • Myosin Heavy Chains / genetics
  • Myosin Heavy Chains / metabolism
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription, Genetic
  • Transcriptional Activation

Substances

  • Actins
  • Carrier Proteins
  • DNA, Complementary
  • EID-2 protein, mouse
  • EID2 protein, human
  • Inhibitor of Differentiation Protein 2
  • Intracellular Signaling Peptides and Proteins
  • MyoD Protein
  • Nuclear Proteins
  • Trans-Activators
  • Acetyltransferases
  • Histone Acetyltransferases
  • Myosin Heavy Chains

Associated data

  • GENBANK/AY251272
  • GENBANK/AY251273