Screening of DNA aptamers inhibiting Taq DNA polymerase using algorithm mimicking evolution

Kazunori Ikebukuro; Takahisa Noma

doi:10.1093/nass/3.1.309

Screening of DNA aptamers inhibiting Taq DNA polymerase using algorithm mimicking evolution

Nucleic Acids Res Suppl. 2003:(3):309-10. doi: 10.1093/nass/3.1.309.

Authors

Kazunori Ikebukuro¹, Takahisa Noma

Affiliation

¹ Department of Biotechnology & Life Science, Tokyo University of Agriculture & Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.

PMID: 14510504
DOI: 10.1093/nass/3.1.309

Abstract

We have developed a novel method for screening DNA aptamers by an algorithm mimicking evolution, which have enabled selection by not affinity but inhibitory activity of aptamers. Using this method, we have successfully obtained DNA aptamers that inhibit Taq DNA polymerase in a few hundred-nanomolar range. This result shows this method would be useful for screening aptamers as an enzyme inhibitor.

MeSH terms

Algorithms*
DNA / chemistry*
DNA / pharmacology
Enzyme Inhibitors / chemistry*
Enzyme Inhibitors / pharmacology
Evolution, Molecular*
Taq Polymerase / antagonists & inhibitors*

Substances

Enzyme Inhibitors
DNA
Taq Polymerase