Abstract
Competition with specific oligonucleotides in DNA-binding experiments, polyacrylamide gel electrophoresis, and recognition by specific antibodies have identified the ubiquitous transcription factor COUP as one of the nuclear proteins binding to the promoter region of the bovine oxytocin gene in endogenously expressing bovine granulosa cells. PCR cloning of partial cDNA sequences for bovine COUP-TF I and II and development of RNase protection assays demonstrated the up-regulation of COUP-TF in bovine granulosa cells and corpus luteum under conditions where the oxytocin gene is switched off. These experimental results from in vitro and in vivo studies point to the direct involvement of COUP-TF in oxytocin gene down-regulation during luteinization of bovine granulosa cells.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Base Sequence
-
Binding Sites
-
COUP Transcription Factor I
-
Cattle
-
Cell Nucleus / metabolism
-
Corpus Luteum / physiology*
-
DNA / genetics
-
DNA / isolation & purification
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Female
-
Gene Expression Regulation*
-
Granulosa Cells / physiology*
-
Humans
-
Molecular Sequence Data
-
Oligodeoxyribonucleotides
-
Oxytocin / biosynthesis
-
Oxytocin / genetics*
-
Polymerase Chain Reaction / methods
-
Ribonucleases
-
Sequence Homology, Nucleic Acid
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
Substances
-
COUP Transcription Factor I
-
DNA-Binding Proteins
-
NR2F1 protein, human
-
Oligodeoxyribonucleotides
-
Transcription Factors
-
Oxytocin
-
DNA
-
Ribonucleases
Associated data
-
GENBANK/D12944
-
GENBANK/D12945
-
GENBANK/D12946
-
GENBANK/D12947
-
GENBANK/D12948
-
GENBANK/D12949
-
GENBANK/D12950
-
GENBANK/D12951
-
GENBANK/S50056
-
GENBANK/S50057