[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli]

M K Chekhranova; E R Shuvalova; A M Kutin; V Iu Butnev; A B Valentsova; E N Il'ina; Iu A Pankov

[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli]

Mol Biol (Mosk). 1992 May-Jun;26(3):596-600.

[Article in Russian]

Authors

M K Chekhranova, E R Shuvalova, A M Kutin, V Iu Butnev, A B Valentsova, E N Il'ina, Iu A Pankov

PMID: 1406615

Abstract

A human insulinoma cDNA library was constructed in expression plasmid vector pUEX1. Clone pUEX1Ins12 was selected from human insulinoma cDNA library by means of hybridization with the insulin probe and a nucleotide sequence of the insertion was determined. It codes for full size amino acid sequence preproinsulin and furthermore, contains the entire 3'-end of noncoding mRNA region and 44 nucleotides from the 5'-untranslated region. The bacterial strain pUEX3Ins8 producing preproinsulin as beta-galactosidase fusion protein was constructed.

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA*
Escherichia coli / genetics*
Gene Expression*
Humans
Insulin
Insulinoma / metabolism*
Molecular Sequence Data
Plasmids
Proinsulin / genetics*
Protein Precursors / genetics*

Substances

Insulin
Protein Precursors
preproinsulin
DNA
Proinsulin