Abstract
The HIV Tat protein is able to upregulate the transcription by RNA polymerase III of cotransfected or endogenous cellular Alu-repeated sequences in both HeLa and Jurkat T cells. This effect is mediated by an increase in the activity of transcription factor TFIIIC, which binds to the B box in the RNA polymerase III Alu promoter. This is the first example of an effect of the Tat protein on the transcription of a cellular gene or on the activity of a cellular transcription factor. The significance of this effect for the life cycle of HIV and its interaction with infected cells is discussed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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Base Sequence
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Cell Line
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DNA, Viral / chemistry
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DNA, Viral / genetics
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Gene Expression Regulation, Viral
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Gene Products, tat / physiology*
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HIV / genetics*
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HeLa Cells
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Humans
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Molecular Sequence Data
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Oligonucleotides / chemistry
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Repetitive Sequences, Nucleic Acid*
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Transcription Factors / physiology*
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Transcription Factors, TFIII*
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Transcription, Genetic*
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Transfection
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tat Gene Products, Human Immunodeficiency Virus
Substances
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Actins
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DNA, Viral
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Gene Products, tat
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Oligonucleotides
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RNA, Messenger
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Transcription Factors
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Transcription Factors, TFIII
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tat Gene Products, Human Immunodeficiency Virus
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transcription factor TFIIIC