Abstract
Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Oxidoreductases / genetics*
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Amino Acid Oxidoreductases / metabolism
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain / enzymology*
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Cell Line
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Cloning, Molecular
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Humans
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Isoenzymes / genetics*
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Isoenzymes / metabolism
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Kinetics
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Macrophages / enzymology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Neurons / enzymology*
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Nitric Oxide Synthase
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Oligonucleotides, Antisense
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Recombinant Proteins / metabolism
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Sequence Homology, Nucleic Acid
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Spleen / enzymology
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Transfection
Substances
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Isoenzymes
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Oligonucleotides, Antisense
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RNA, Messenger
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Recombinant Proteins
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Nitric Oxide Synthase
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Amino Acid Oxidoreductases