Cloned and expressed macrophage nitric oxide synth...[Proc Natl Acad Sci U S A. 1992]

SearchDatabase nameforSearch term

Advanced Search

Your browser version may not work well with NCBI's Web applications. More information here...

Display Show

All: 1

Review: 0

Click to change filter selection through MyNCBI.

1: Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6711-5. Links

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

Lowenstein CJ, Glatt CS, Bredt DS, Snyder SH.

Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, MD 21205.

Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.

PMID: 1379716 [PubMed - indexed for MEDLINE]

PMCID: PMC49573

Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. [Proc Natl Acad Sci U S A. 1993]
Molecular cloning and functional expression of an inducible nitric oxide synthase from a murine macrophage cell line. [J Biol Chem. 1992]
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. [Nature. 1991]
Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. [Science. 1992]
ReviewNitric oxide: a physiologic messenger molecule. [Annu Rev Biochem. 1994]

Display Show

Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme.

Related Articles